This dataset consists of 11 single-source samples which were genotyped/sequenced with assays targeting Forensic DNA markers. The CE-STR assays reported are: Applied Biosystems GlobalFiler, Applied Biosystems Y-Filer Plus, Promega PowerPlex Fusion 6C, Promega PowerPlex Y23. The CE profiles are also included in a spreadsheet; more information can be found in the Readme_CE text file. Sequencing assays reported include the PowerSeq CRM Nested System (mitochondrial DNA control region sequence, additional information can be found in the Readme_mtDNA text file) and the Verogen ForenSeq DNA Signature Prep Kit (STR, Y-STR, X-STR, SNP sequences). This dataset is intended for educational purposes only. This work was approved by the NIST Research Protections Office. NOTE: Files ending in "sha256" serve as digital signatures for the associated files; however, the actual data is contained in the files without this suffix.