This dataset consists of single source and mixture samples which were genotyped/sequenced with assays targeting Forensic DNA markers. The CE-STR assays reported for the single source samples include: Applied Biosystems GlobalFiler, Applied Biosystems Y-Filer Plus, Promega PowerPlex Fusion 6C, Promega PowerPlex Y23. The CE profiles are also included in a spreadsheet; more information can be found in the Readme_CE text file. Sequencing assays reported include the PowerSeq CRM Nested System (single source samples only, mitochondrial DNA control region sequence, additional information can be found in the Readme_mtDNA text file) and the Verogen ForenSeq DNA Signature Prep Kit (single source and mixture samples containing STR, Y-STR, X-STR, SNP sequences, additional information can be found in the Readme_SignaturePrep text file). This dataset is intended for educational purposes only. This work was approved by the NIST Research Protections Office. Certain commercial equipment, instruments, or materials are identified in this dataset in order to specify the experimental procedure adequately. Such identification is not intended to imply recommendation or endorsement by NIST, nor is it intended to imply that the materials or equipment identified are necessarily the best available for the purpose.